WIV: RaTG15 and seven other coronaviruses from bats in Mojiang cannot use human ACE2 as entry receptor

News (1) 

RaTG15 and seven other coronaviruses from bats in Mojiang cannot use human ACE2 as entry receptor

Report by : Gan Yung Chyan, KUCINTA SETIA / Image credit : Shi Zhengli etal, bioRxiv

The viologists of Wuhan Institute of Viology (WIV) have published their latest research on other bat coronaviruses in Tongguan town, Mojiang county, Yunnan, that they discover in May 2015. Their findings appear in a peer-unreviewed paper entitled "Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor" on the Biology preprint server bioRxiv. 

Mojiang is the location where Shi Zhengli discovered bat coronavirus RaTG13 in 2013 in the cave, not 2012.

Shi Zhengli's principal Wang Yanyi claims that the Wuhan Institute of Viology (WIV) does not have live virus of RaTG13, whose sequence forms the genome of SARS-CoV-2 that the WIV viologists first shared with the world's infectious disease experts, hence SARS-CoV-2 WIV04 in full.

According to Shi Zhengli and Zhou Peng, RaTG15 and related viruses share 97.2% amino sequence identifies to SARS-CoV-2 in the conserved ORF1b region, but only show less than 77.6% to SARS-CoV and SARS-CoV-2 in genome level. The RaTG15 receptor binding domain (RBD) can bind to and use the intermediate horseshoe bat ACE2 but not human ACE2 as entry receptor.

Intermediate horseshoe bats (Rhinolophus affinis) are found in Southeast Asia (Thailand, Malaysia, Pulau Ubin, Pulau Tekong, Indochina, Indonesia and Myanmar), southwest China, southern China and eastern China, Bangladesh, Bhutan, Nepal and northern India.

This proves that the intermediate horseshoe bats are not hosts of SARS-CoV-2.

The viologists also detected a coronavirus from Malayan horsehose bat (Rhinolophus malayanus), RmYN02, which contains a similar insertion at the S1/S2 cleavage site in the spike of SARS-CoV-2 but it has some deletions in the RBD and fails to bind with human ACE2.

Shi Zhengli etal argued that Pangolin-CoV-GD, a coronavirus derived from Malayan pangolin (Manis javanica) possesses six identical critical residues of RBD with SARS-CoV-2 and displays a similar binding affinity to human ACE2 compared with SARS-CoV-2 but it does not mean that the pangolin is the intermediate host of SARS-CoV-2 as it shows lower sequence identity to SARS-CoV-2 in genome compared with RaTG13.

Failure to determine the actual host of SARS-CoV-2 despite years of research supports the higher possibility of human intervention in creating SARS-CoV-2 in CCP China.

Refs: https://www.biorxiv.org/content/10.1101/2021.05.21.445091v1.full.pdf

https://staygate.blogspot.com/2021/04/wuhan-laboratory-staff-cooperated-with.html, https://staygate.blogspot.com/2021/04/sars-cov-2-originates-from-chinese.html, https://staygate.blogspot.com/2021/03/patient-zero-of-covid-in-september-2019.html, https://staygate.blogspot.com/2021/02/yan-limeng-chinese-academy-of-military.html, https://staygate.blogspot.com/2021/01/chinese-netizens-chinese-communist.html, https://staygate.blogspot.com/2021/01/pompeo-researchers-of-wuhan-institute.html, https://staygate.blogspot.com/2021/01/when-did-sars-cov-2-overflow-to-humans.html, https://staygate.blogspot.com/2020/10/yan-limengs-second-report-sars-cov-2-is.html, https://staygate.blogspot.com/2020/08/shi-zhengli-disputes-bats-carry-sars.html, https://staygate.blogspot.com/2020/07/did-china-discover-sars-cov-2-in-2013.html, https://staygate.blogspot.com/2020/09/yan-limengs-heavy-report-analysis-3-key.html

News (2)

Is the laboratory virus leak a conspiracy theory? U.S. host criticizes the media for misleading the public

Reporter : Li Zhaoxi / Editor : Li Jia / https://www.ntdtv.com/gb/2021/05/26/a103128249.html / Direct translation

Image : Wuhan Institute of Virology, Chinese Academy of Sciences (Wuhan P4 Laboratory). (Hector Retamal, Johannes Eisele / AFP via Getty Images)

 Recently, there has been new evidence about the laboratory leak theory of the origin of SARS-CoV-2 (CCP virus) / COVID-19 (covid). Fox News host Greg Gutfeld criticized the media for portraying the laboratory leak theory as a conspiracy theory, causing people to disbelieve this theory and causing heavy losses.

Gutfeld said in the "The Five" program aired on Tuesday (25th) that reporters drove people to ignore the most obvious answer because they were persuaded by their hatred of former President Trump. He thinks that what Trump believes cannot be true. Their emotional aversion to Trump actually prevents them from thinking about this issue logically.

Gutfield pointed out that the laboratories on the streets of Wuhan are doing experiments on the new type of CCP virus. As we all know, they are not fully competent in hygiene. They are known for their sloppy work, and they are actually likely to allow the virus to escape. This is obviously a more reasonable theory, "but we are all bullied by the media."

"When you say this, think about it, China murdered my father-in-law. Everyone you know died, was murdered, and changed my life forever, and changed my wife's life forever." Gutfeld added, "They are murderers. That government is a murderous government. We know this, so what are we going to do? I don't know."

Senator Tom Cotton, Republican of Arkansas, has publicly urged the Biden administration to hold the CCP accountable for the possible source of SARS-CoV-2. Cotton told the host Maria Bartiromo on the Sunday Morning Futures program of the Fox News Channel that there are various signs that the CCP virus originated in a laboratory in Wuhan, not like a CCP government agency as claimed.

Cotton said, "Maria, it’s hard to believe that 15 months have passed since you and I first discussed what I think is a very common-sense proposition for most Arkansas. This is definitely a big one. Coincidentally, this virus did not originate from some remote mountain villages full of bat caves, but in the centre of Wuhan, a city bigger than New York, only a few blocks away from the laboratory for bat coronavirus research."

Cotton also pointed out that some of the evidence subsequently discovered, even in the last few days of the Trump administration, continue to point to the Wuhan laboratory as the origin of the CCP virus, not the food that the CCP used as a cover from the beginning. market.

"This is why the Biden administration must hold the CCP accountable and require it to answer all these questions," Cotton said.

Howard Husock, a senior researcher at the Manhattan Institute, a conservative think tank in New York and a special editor of the quarterly magazine City Journal, believes that "confidentiality" is the norm in the Chinese Communist regime As long as the Chinese Communist Party rules this country, the future world will never be immune to pandemics.