Reporter : Xing Yu
Publisher : Chinese Science News
Ref : http://news.sciencenet.cn/htmlnews/2020/5/440680.shtm
Translation, editing : Gan Yung Chyan
/ KUCINTA SETIA
On 27 May 2020, Academician Chen Wei of the Institute of Bioengineering, Academy of Military Medical Sciences, Academy of Military Sciences, Wang Xiaoqun, a researcher of the Institute of Biophysics, Chinese Academy of Sciences, and Wu Qian, a researcher of the State Key Laboratory of Cognitive Neuroscience and Learning at Beijing Normal University, posted on the preprint website MedRxiv published the latest research results. The researchers through single-cell RNA sequencing and V (D) J recombination analysis revealed the transcription profile and changes of immune cells in patients with new coronary pneumonia (covid).
Covid is an ongoing global health problem. At present, the immunopathological changes of key types of immune cells during and after infection with SARS-CoV-2 are not clear.
The researchers enriched CD3 + and CD19 + lymphocytes from the peripheral blood mononuclear cells of patients with covid (severe patients, early or late rehabilitation patients) and healthy people, and through comprehensive single cell RNA sequencing and V (D) J recombination analysis to study the transcription profiles and changes of these lymphocytes.
The researchers found that although the number of T lymphocytes in the blood of people infected with covid is reduced, the remaining T cells still have high expression of inflammatory genes. And within a period of time after the patient recovered, the researchers also observed the potential transformation of CD8 effector T cells into central memory T cells.
In B lymphocytes, the researchers analyzed the expansion trajectory of a class of plasma cell subtypes in patients with severe covid and traced its origin to atypical memory B cells (AMBC).
Further sequencing analysis of B-cell and T-cell receptors showed that there is a high level of clonal expansion in patients with severe new coronary pneumonia, especially clonal expansion of B lymphocytes, and the high expression of these B cells is related to inflammation and lymphocyte activation gene. In addition, the researchers also summarized the V-J gene rearrangement and high-frequency clone types in patients with covid.
In summary, the results of this study provide important insights into the immune response of patients with severe covid and rehabilitation patients, and provide useful information for the further development of SARS-CoV-2 vaccines and research treatment strategies.
Related paper information: https://www.medrxiv.org/content/10.1101/2020.05.24.20101238v1
Publisher : Chinese Science News
Ref : http://news.sciencenet.cn/htmlnews/2020/5/440680.shtm
Translation, editing : Gan Yung Chyan
/ KUCINTA SETIA
On 27 May 2020, Academician Chen Wei of the Institute of Bioengineering, Academy of Military Medical Sciences, Academy of Military Sciences, Wang Xiaoqun, a researcher of the Institute of Biophysics, Chinese Academy of Sciences, and Wu Qian, a researcher of the State Key Laboratory of Cognitive Neuroscience and Learning at Beijing Normal University, posted on the preprint website MedRxiv published the latest research results. The researchers through single-cell RNA sequencing and V (D) J recombination analysis revealed the transcription profile and changes of immune cells in patients with new coronary pneumonia (covid).
Covid is an ongoing global health problem. At present, the immunopathological changes of key types of immune cells during and after infection with SARS-CoV-2 are not clear.
The researchers enriched CD3 + and CD19 + lymphocytes from the peripheral blood mononuclear cells of patients with covid (severe patients, early or late rehabilitation patients) and healthy people, and through comprehensive single cell RNA sequencing and V (D) J recombination analysis to study the transcription profiles and changes of these lymphocytes.
The researchers found that although the number of T lymphocytes in the blood of people infected with covid is reduced, the remaining T cells still have high expression of inflammatory genes. And within a period of time after the patient recovered, the researchers also observed the potential transformation of CD8 effector T cells into central memory T cells.
In B lymphocytes, the researchers analyzed the expansion trajectory of a class of plasma cell subtypes in patients with severe covid and traced its origin to atypical memory B cells (AMBC).
Further sequencing analysis of B-cell and T-cell receptors showed that there is a high level of clonal expansion in patients with severe new coronary pneumonia, especially clonal expansion of B lymphocytes, and the high expression of these B cells is related to inflammation and lymphocyte activation gene. In addition, the researchers also summarized the V-J gene rearrangement and high-frequency clone types in patients with covid.
In summary, the results of this study provide important insights into the immune response of patients with severe covid and rehabilitation patients, and provide useful information for the further development of SARS-CoV-2 vaccines and research treatment strategies.
Related paper information: https://www.medrxiv.org/content/10.1101/2020.05.24.20101238v1
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